cDNA encoding the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei.
نویسندگان
چکیده
VSG lipase of Trypanosoma brucei specifically cleaves the glycosyl-phosphatidylinositol membrane anchor of the trypanosome variant surface glycoprotein (VSG), releasing this protein from the plasma membrane. It also cleaves similar membrane anchors on some mammalian proteins. VSG lipase may play a role in processes such as parasite differentiation or antigenic variation. We describe here the cloning and sequencing of a cDNA encoding VSG lipase from T. brucei.
منابع مشابه
Intracellular localization of the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei.
Glycosyl-phosphatidylinositol-specific phospholipase C (GPI-PLC) is a membrane-bound enzyme of bloodstream forms of Trypanosoma brucei, which cleaves the GPI-membrane anchor of the variant surface glycoprotein forming diacylglycerol and 1,2-cyclic phosphate on the inositol ring. The cellular localization of the enzyme was studied by fractionation of sub-cellular organelles and immunofluorescenc...
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متن کاملExpression of bloodstream variant surface glycoproteins in procyclic stage Trypanosoma brucei: role of GPI anchors in secretion.
Using transformed procyclic trypanosomes, the synthesis, intracellular transport and secretion of wild-type and mutant variant surface glycoprotein (VSG) is characterized. We find no impediment to the expression of this bloodstream stage protein in insect stage cells. VSG receives a procyclic-type phosphatidylinositol-specific phospholipase C-resistant glycosyl phosphatidylinositol (GPI) anchor...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 85 23 شماره
صفحات -
تاریخ انتشار 1988